Industrial Technology Research Institute

Technology Overview

ITRI developed a dendritic cell cancer vaccine therapy by employing an mRNA coding for a soluble multimeric CD40 ligand fused with a shared MHC class I-neoantigen peptide, along with lymph node-targeting nanoparticles to deliver the mRNA payload to dendritic cells in mice through intravenous injection.

Features & Specifications

Features

• Compared with tumor cell-based and peptide vaccines, mRNA has the advantage of rapid production and direct priming of T cells by DCs.
• The adjuvant effect of multimeric CD40L can induce a long-lasting immune cell responses.
• Next-generation sequencing and AI big data analysis facilitate personalized cancer vaccine development.

Specifications

• Multimeric CD40L–CD40 clustering elicits strong downstream signaling that promotes dendritic cell maturation and activation, enhancing IL-12 production and driving CD8⁺ T cell activation and cytotoxicity.
• Multimeric CD40L fused to tumor-specific antigens and delivered into dendritic cells via DNA or mRNA markedly improves antigen presentation and CD8⁺ T cell activation compared to conventional vaccines, enabling reduced antigen dosage and cost.
• Multimeric CD40–CD40L interactions can bypass the requirement for CD4⁺ helper T cells and promote the differentiation of activated CD8⁺ T cells into memory CD8⁺ T cells, thereby conferring durable protective immunity.
• The soluble multimeric CD40L can significantly reduce cytotoxicity compared to the transmembrane form or anti-CD40 antibodies.

Applications & Benefits

• Ex vivo pulsed and activated dendritic cell vaccination against cancer.
• mRNA-loaded lipid nanoparticles targeting dendritic cells for cancer immunotherapy.
• Multimeric CD40L fusions as a potent immunological adjuvant to increase antibody titer.

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