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工業技術研究院

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技術名稱: 小鼠抗體人源化與親和力增進技術

技術簡介

該技術係先固定小鼠抗體之重鏈或輕鏈,配合可變之人類輕鏈或重鏈基因組,針對特定標靶蛋白篩選得到最適之人類輕鏈或重鏈結合者後,再固定之,並將另一鏈改為可變的重鏈或輕鏈,用相同方法篩選,最後可得到一完全擬人化的抗體

Abstract

This is an antibody humanization technique which involves retaining murine heavy/light chain variable region and shuffling the light/heavy chain variable region with human antibody library. After obtaining the best binder by phage display, retain the binder and shuffle the other chain. As a result, a human new antibody is selected out.

技術規格

可快速將抗體片段擬人化同時保留高專一性,並有親和力增進的效果。

Technical Specification

A fast antibody humanization and affinity maturation technique without losing the specificity

技術特色

篩選時保留部分之小鼠抗體片段,可在維持一定親和力的狀況下進行篩選,增加篩選效率。

應用範圍

作為快速篩選有潛力之診斷或治療用擬人化抗體之用,進而得到高專一性和高親和力的抗體,並降低在人體內被分解以及引發免疫反應 (HAMA, human anti-mouse antibody response)的風險。

接受技術者具備基礎建議(設備)

ELISA reader、FPLC、細菌與細胞培養設備

接受技術者具備基礎建議(專業)

噬菌體展現技術,分子選殖技術,蛋白質純化技術,細胞培養技術

技術分類 分子診斷

聯絡資訊

聯絡人:周民元 蛋白質工程研究室

電話:+886-3-5912144 或 Email:minyuanc@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5910097

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