『您的瀏覽器不支援JavaScript功能,若網頁功能無法正常使用時,請開啟瀏覽器JavaScript狀態』

跳到主要內容區塊

工業技術研究院

:::

技術名稱: 基因轉殖神經退化疾病動物模式表現TRKA融合蛋白

技術簡介

利用分子生物學技術產製具有神經退化性疾病的轉殖基因動物,此基因轉殖動物係表現神經滋養因子受器(TrkA)膜外蛋白構造與人類免疫球蛋白Fc構造的融合蛋白質,藉此中和神經滋養因子的表現,並產生神經退化的病徵,成為神經退化的疾病動物模式;此基因轉殖動物可應用於神經退化性疾病藥物開發之篩選與評估、藥物作用機制探討,與神經退化疾病基礎科學研究之工具平台。

Abstract

The present invention provides a neurodegeneration, non-human transgenic mammal whose cells contain a recombinant DNA sequence (the TrkA-hFc transgene) for encoding at least a TrkA fusion protein or a defective TrkA receptor. This invention can be useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments foe various disease states and disease conditions.

技術規格

表現TrkA膜外蛋白構造與人類免疫球蛋白Fc構造的融合蛋白質

Technical Specification

Animals contain a recombinant DNA sequence for encoding at least a TrkA-hFc fusion protein.

技術特色

神經生長因子(Neurotrophin nerve growth factor, NGF) 參與老化相關的神經退化性疾病,剔除神經生長因子的基因轉殖小鼠會造成死亡,因此無法進行神經生長因子在成鼠上的功能探討研究。本創作即利用基因工程技術產製表現TrkA-trap的基因轉殖鼠,藉由中和多種經由TrkA進行訊息傳導的neurotrophin的作用,使得基因轉殖鼠出現人類神經退化疾病病徵。目前已成功產製TrkA-trap的基因轉殖鼠,並以病理切片與記憶行為試驗完成此基因轉殖鼠具神經退化病徵的驗證。

應用範圍

建立神經退化疾病動物模式,作為前臨床神經退化藥物篩選、藥效評估、藥物作用機制探討與神經退化疾病基礎科學研究之關鍵技術平台。

接受技術者具備基礎建議(設備)

分子生物學與基因轉殖相關設備,動物飼養廠房

接受技術者具備基礎建議(專業)

需具備分子生物學、生物化學之知識,與動物轉殖基因之專業經驗

技術分類 藥物治療

聯絡資訊

聯絡人:劉志鵬 小分子藥物技術組

電話:+886-3-5732620 或 Email:cpliu@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5743990

[{"text":"企業網","weight":13.0},{"text":"材化所","weight":11.5},{"text":"機械所","weight":10.0},{"text":"綠能所","weight":9.4},{"text":"生醫所","weight":8.0},{"text":"半導體","weight":6.2},{"text":"南分院","weight":5.0},{"text":"太陽能","weight":5.0},{"text":"課程","weight":5.0},{"text":"遠紅外線","weight":5.0},{"text":"雷射","weight":4.0},{"text":"LED","weight":4.0},{"text":"LED可見光","weight":3.0},{"text":"5G","weight":3.0},{"text":"工研人","weight":3.0},{"text":"電光所","weight":3.0},{"text":"綠能與環境研究所","weight":3.0},{"text":"機械","weight":3.0},{"text":"資通所","weight":2.0},{"text":"面板","weight":2.0},{"text":"文字轉語音","weight":2.0},{"text":"實習","weight":2.0},{"text":"無人機","weight":2.0},{"text":"生醫","weight":2.0},{"text":"3D","weight":2.0},{"text":"v2x","weight":2.0},{"text":"員工","weight":2.0},{"text":"地圖","weight":2.0},{"text":"太陽光電","weight":2.0},{"text":"材料與化工研究所","weight":1.0}]