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工業技術研究院

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技術名稱: Mature miRNA純化技術

技術簡介

以電泳膠搭配可吸附核酸之帶電固體介質,進行核酸純化。本技術特色為流程簡單,並可與自動化儀器整合之核酸純化方法。

Abstract

The nucleic acids purification method is a combination of electrophoresis gel and charged solid phase. The purification process is simple and can be integrated with automation.

技術規格

可視使用者需求,選擇純化mature miRNA(4~40 nt)或miRNA precursor (40~100 nt)的片段範圍,可於生物晶片獨立觀察兩者表現量。

Technical Specification

Mature miRNAs (4~40 nt) or miRNA precursors (40~100 nt) can be isolated effectively. The purified product can be applied to microarray right after the labeling.

技術特色

目前市面上並無可將miRNA清楚切割分離成mature miRNAs及miRNA precursors兩群的純化產品,使得miRNA生物晶片這類high-throughput screening tools無法用於觀察這兩群miRNA含量對生物作用之影響。目前僅有Agilent公司利用特殊設計之探針,可專一讀取mature miRNA之訊號,但仍無法觀察miRNA precursors。

應用範圍

miRNA microarray相關應用

接受技術者具備基礎建議(設備)

1.核酸純化試劑生產設備 2.RNase-free耗材生產環境

接受技術者具備基礎建議(專業)

1.分生試劑生產經驗 2.RNase-free耗材生產製程經驗

技術分類 藥物治療

聯絡資訊

聯絡人:陳廷碩 體外診斷醫材技術組

電話:03-5912143 或 Email:CTS@itri.org.tw

客服專線:+886-800-45-8899

傳真:03-5912444

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