工業技術研究院

技術簡介

非酒精性脂肪肝病（Non-alcoholic fatty liver disease, NAFLD）為肝臟中三酸甘油脂的異常堆積是世 界上最常見的慢性肝病，其關鍵致病因素是肥胖症、代謝 症候群，胰島素抗性和2型糖尿病等。NAFLD的肝臟組織病 變依病程進展包含了簡單的脂肪肝、非酒精性脂肪性肝炎 （Non-alcoholic steatohepatitis, NASH）到最終纖 維化之肝硬化，甚至可發展為肝癌。本技術使用雄性 BKS.Cg- Dock 7m +/+ Leprdb/JNarl (db/db)小鼠建 立非酒精性脂肪肝病動物模型以了解其複雜的致病機制理 解及合適的治療策略。

Abstract

Non-alcoholic fatty liver disease（NAFLD）, the abnormal accumulation of triglycerides in the liver, is the most common chronic liver disease worldwide. NAFLD includes a spectrum of histopathological findings, ranging from simple fatty liver through non-alcoholic steatohepatitis（NASH） to fibrosis and ultimately cirrhosis, which may progress to hepatocellular carcinoma. The key pathogenetic factors of NAFLD are obesity, metabolic syndrome, insulin resistance and type 2 diabetes mellitus. We used db/db mice to apply the animal model for this disease to improve the understanding of its complex pathogenesis and to discover appropriate therapeutic strategies.

技術規格

以小鼠餵食高熱量食物建立之非酒精性脂肪肝疾病/非酒精 性肝炎動物模式，5至15週後能成功誘導NAFLD和NASH，並 且與人類有相似的血清生化結果及肝臟的組織病理病變。

Technical Specification

Mice were fed with high fat diet to induce NAFLD / NASH. Results showed similar serum biochemistry and histopathologic patterns of human NAFLD/NASH.

技術特色

已動物模式建立，可進行分析待測樣品對小鼠之非酒精性脂肪肝疾病/非酒精性肝炎之影響

應用範圍

生技醫藥、健康食品效力驗證及機制探討

接受技術者具備基礎建議（設備）

動物飼養及試驗操作之基本環境

接受技術者具備基礎建議（專業）

實驗動物操作及觀測等專業

聯絡資訊