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工業技術研究院

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技術名稱: 手持式核酸擴增快速分子診斷系統

技術簡介

此小型化的手持式系統,跳脫傳統龐大加熱模組方式,改 以創新機構設計搭配溫度控制手段實現聚合?鏈鎖反應 (PCR),不需要移動物件或其他複雜控制裝置,即可有效 率地達到PCR條件,並研發專屬的光學即時偵測模組,可 達到定性或半定量快速檢測,最快可於30分鐘內得到定性 判別結果。未來適用於各醫護點,可結合試劑開發發展各 類分子診斷應用。

Abstract

Instead of the regular Polymerase Chain Reaction (PCR) systems with thermocycler for heating and cooling, ITRI uses innovative heating control methodology to fulfill rapid temperature control and perform both DNA and RNA amplification with a portable and easy-to- operate PCR system. With an innovative designed optical detection module, the whole system can achieve qualitative or semi- quantitative rapid detection for PCR within 30 minutes. In the near future, this system can be applied to clinics, agricultural testing and aquaculture testing.

技術規格

系統規格 -可偵測樣本數: 6 -樣本體積: 50μl -反應容器: 塑膠毛細管 -觸控面板: 有 -光源: 藍光LED -偵測器: 極靈敏光電二極管 -尺寸: 9×9×15cm (長x寬x高) -重量: 596g (不含電池) -偵測類型: 螢光發光 (SYBR/FAM, VIC)

Technical Specification

System specification -Number of wells: 6 -Sample Volume: 50μl -Reaction tube: Plastic capillary tube -Touchscreen: Yes -Optical Source: Blue LED array -Detector: Ultra sensitive photodiodes -Dimensions: 9 × 9 × 15cm (W x L x H) -Weight: 596g (excluding battery) -Detection: Fluorescence (SYBR/FAM, VIC)

技術特色

-小型化PCR反應暨偵測裝置,取代目前市場上的大型機台 -PCR反應放大時間: < 30分鐘,樣本可不需要特別預熱 -能同時分析兩種螢光的反應變化量 -簡易操作之人機介面系統 -能在同一裝置實現DNA與RNA的擴增

應用範圍

-可結合試劑開發,發展各類POCT與分子診斷應用 -傳染性疾病快速分子診斷 -水產、畜產動物疾病快速分子診斷

接受技術者具備基礎建議(設備)

none

接受技術者具備基礎建議(專業)

光機電整合、分子檢測及診斷技術相關

技術分類 分子診斷

聯絡資訊

聯絡人:江佩馨 精準醫療指引診斷技術組(0N000)

電話:+886-3-5912526 或 Email:pspeggy@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5912444

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