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工業技術研究院

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技術名稱: 螢光金奈米簇製備方法及含硫醇基物質的檢測方法

技術簡介

本發明揭示一種金奈米簇組成物及其製備方法,包含提供金離子溶液; 混合金離子溶液與還原劑溶液,以得第一混合液;加熱第一混合液,以得第二混合液,其中第二混合液含金奈米簇,且還原劑以非飽和之型態擔載於金奈米簇之表面。

Abstract

Gold nanocluster composition and method for preparing the same are provided. The method includes providing a gold ion-containing solution. Next, mixing the gold ion-containing solution and a reducing agent solution to obtain a first mixture liquid; heating the first mixture liquid to obtain a second mixture liquid, wherein the second mixture liquid contains gold nanoclusters, and the reducing agent is non-saturated capped on a surface of the gold nanoclusters.

技術規格

1.本發明提供之金奈米簇組成物,包含還原劑以非飽和之型態擔載(capping)於金奈米簇的表面,且金奈米簇組成物於600-650nm及800-850nm含有螢光放光峰。 2.本發明提供之金奈米簇組成物,包含還原劑以非飽和之型態擔載(capping)於該金奈米簇的表面,且金奈米簇組成物於800-900nm具有單一螢光放光峰。 3.本發明提供之金奈米簇組成物的製備方法,包含:混合一金離子溶液與一還原劑溶液,以得一第一混合溶液;加熱該第一混合溶液,以得一第二混合溶液,其中該第二混合溶液含金奈米簇,其中該還原劑以非飽和之型態擔載(capping)於金奈米簇表面以得到金奈米簇組成物。 4.本發明提供之含硫醇基之物質的檢測方法,包括:提供上述之金奈米簇組成物;提供待測物與金奈米簇組成物反應;以及分析反應結果,以確認待測物是否含硫醇基。

Technical Specification

1.One embodiment of the disclosure provides a gold nanocluster composition, comprising: a gold nanocluster is partially capped by reducing agent, and the gold nanocluster composition has fluorescence emission peaks at 600-650 nm and 800-850 nm. 2.One embodiment of the disclosure provides a gold nanocluster composition, comprising: a gold nanocluster is partially capped by reducing agent, and the gold nanocluster composition has a single fluorescence emission peak at 800-900 nm. 3.One embodiment of the disclosure provides a method for preparing a gold nanocluster composition, comprising: mixing a gold ion-containing solution and a reducing agent solution to obtain a first mixture liquid; and heating the first mixture liquid to obtain a second mixture liquid, wherein the second mixture liquid contains a gold nanocluster composition, and the gold nanoclusters are partially capped by reducing agent. 4.One embodiment of the disclosure provides a method for detecting a thiol-containing compound, including: providing the described gold nanocluster compositions; providing an analyte to react with the gold nanocluster compositions; and analyzing the reaction result to check whether the analyte contains a thiol-containing compound or not.

技術特色

金奈米簇由於較半導體螢光量子點的毒性小,同時具高光穩度性且合成步驟簡單等優點,因此具有發展成為新穎生物螢光標記及生物感測器的潛力。然而現有合成金奈米簇及表面修飾方法反應時間長且步驟繁瑣。若是將生物分子固定於金奈米簇上,更需要繁複且耗時的實驗步驟。基於上述,業界需要合成簡易的金奈米簇,同時無需透過化學反應修飾,就能即時檢測含硫醇基物質之技術。本發明主要是一種可自發螢光的金奈米簇材料及其製造方法,在適當的條件下可製造出表面的部分面積修飾有麩胱甘? (Glutathione, GSH) 分子的金奈米簇,當含有硫醇之小分子或胜?水溶液與金奈米簇水溶液混合時,會直接鍵結於金奈米簇未修飾之表面,造成金奈米簇原始螢光光譜發生變化,而可進行即時偵測,亦可做為製造功能性金奈米簇探針的平台。

應用範圍

臨床檢測、環境分析

接受技術者具備基礎建議(設備)

微波或加熱設備, 螢光分析設備

接受技術者具備基礎建議(專業)

化學合成, 光譜分析

技術分類 分子診斷

聯絡資訊

聯絡人:陳振泰 精準醫療指引診斷技術組(0N000)

電話:+886-3-5918387 或 Email:ivdteddy@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5912444

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