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工業技術研究院

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技術名稱: 金屬離子與化學/生化分子的偵測方法

技術簡介

本發明提供之偵測金屬離子與化學/生化分子的方法,包括:提供探針,且探針包括:金奈米簇;以及還原劑與螯合劑,擔載於金奈米簇的表面,其中探針係由還原劑還原金離子而成,且金離子與還原劑的莫耳比例介於1:0.7至1:1.9之間。探針能夠與水溶液中的多種金屬離子作用產生不同的螢光光譜變化,並且藉由化學/生化分子與金屬離子交互作用所造成的螢光差異偵測化學/生化分子。

Abstract

Disclosed is a method of detecting metal ions and chemical and biochemical molecules, including: providing a probe including gold nanoclusters capped with reducing agents and chelating agents, wherein the probe is prepared by reducing gold ions in the presence of the reducing agents, and the gold ions and the reducing agents have a molar ratio of 1:0.7 to 1:1.9. The probe is capable of interacting a variety of metal ions in aqueous solution and lead to the change of the fluorescence spectrum. Chemical and biochemical molecules interact with metal ions can further result in the change of fluorescence properties and be detected.

技術規格

1. 本發明提供之探針,包括:螢光性金奈米簇組合物;其中還原劑擔載於螢光性金奈米簇的表面,其中螢光性金奈米簇係由還原劑還原金離子而成,且金離子與還原劑的莫耳比例介於1:0.7至1:1.9之間。 2. 本發明提供之偵測金屬離子的方法,包括:提供探針,且探針包括:螢光性金奈米簇組合物;其中還原劑擔載於螢光性金奈米簇的表面,其中螢光性金奈米簇係由還原劑還原金離子而成,且金離子與還原劑的莫耳比例介於1:0.7至1:1.9之間;以及混合探針與待測溶液以形成混合物,藉由探針之螢光光譜與混合物之螢光光譜差異,分析待測溶液中是否含有特定金屬離子。 3. 本發明提供之偵測化學/生化分子的方法,包括:提供探針,且探針包括:螢光性金奈米簇組合物;其中還原劑擔載於螢光性金奈米簇的表面;以及金屬離子,螯合至還原劑,其中螢光性金奈米簇係由還原劑還原金離子而成,其中金離子與還原劑的莫耳比例1:0.7至1:1.9之間;以及混合探針與待測溶液以形成混合物,藉由探針之螢光光譜與混合物之螢光光譜差異,分析待測溶液是否含有特定化學/生化分子。

Technical Specification

1. One embodiment of the disclosure provides a probe, comprising: a gold nanocluster; and a reducing agent partially capped on a surface of the gold nanocluster; wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9. 2. One embodiment of the disclosure provides a method for detecting metal ions, comprising: providing a probe, wherein the probe includes: a gold nanocluster; and a reducing agent partially capped on a surface of the gold nanocluster, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9; and mixing the probe and an analyte solution to form a mixture, and analyzing the analyte solution to determine whether it contains specific metal ions or not by comparing the fluorescent spectra difference of the probe and the mixture. 3. One embodiment of the disclosure provides a method for detecting chemical/biochemical molecules, comprising: providing a probe, wherein the probe includes: a gold nanocluster; a reducing agent partially capped on a surface of the gold nanocluster; and metal ions chelated to the reducing agent, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9; and mixing the probe and an analyte solution to form a mixture, and analyzing the analyte solution to determine whether it contains specific chemical/biochemical molecules or not by comparing the fluorescent spectra difference of the probe and the mixture.

技術特色

開發快速檢測金屬離子的分析方法是當前迫切的需求,傳統金屬離子的偵測方法是使用原子吸收光譜法、感應耦合電漿質譜法、原子螢光法、化學滴定法、電化學分析法以及比色法,這些方法普遍存在有儀器價格昂貴、樣品需求量大、樣品前處理步驟複雜及無法即時偵測的缺點,因此,開發一種微量、簡單、快速、高效的金屬離子檢測方法,並且進一步應用於檢測生化分子,有著重大的實用意義和市場前景。本發明是一種利用螢光性金奈米簇做為金屬離子的感測探針,含有不同金屬離子的水溶液與螢光性金奈米簇混合後會造成螢光光譜產生不同的變化而達到偵測的目的。本發明可做為多重金屬離子的螢光感測探針,亦可將螢光性金奈米簇與金屬離子形成的錯合物視為探針,當含有化學/生化分子的水溶液與錯合物混合後,化學/生化分子會與錯合物中的金屬離子相互作用,造成螢光放光光譜產生變化而可被即時偵測。

應用範圍

臨床檢測、環境分析

接受技術者具備基礎建議(設備)

微波或加熱設備, 螢光分析設備

接受技術者具備基礎建議(專業)

化學合成, 光譜分析

技術分類 分子診斷

聯絡資訊

聯絡人:陳振泰 精準醫療指引診斷技術組(0N000)

電話:+886-3-5918387 或 Email:ivdteddy@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5912444

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