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工業技術研究院

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技術名稱: 人類骨髓間質幹細胞分離培養凍存技術

技術簡介

由骨髓組織分離、培養、凍存、解凍與鑑定人類間質幹細胞之技術

Abstract

Cell isolation, culturing, cryopreservation, and thawing technology for human bone marrow mesenchymal stem cells

技術規格

由50至250ml的骨髓,可分離出間質幹細胞MSC,並以ISCT2006公布的定義分析cell marker CD105,73,90>95%,CD45,34<2%,且具osteoblasts,adipocytes,chondrocytes的分化能力

Technical Specification

Technology for isolation, culturing and cryopreservation of mesenchymal stem cells (MSC) from 50~250ml bone marrow. These cells expressed high level of cell marker CD105,73,90(>95%) and very low CD45,34 (<2%). In addition these cells can differentiated into tri-lineage: osteoblasts, adipocytes and chondrocytes.

技術特色

由骨髓組織分離、培養、凍存、解凍與鑑定人類間質幹細胞之技術,已於一般實驗室,由年輕捐贈者之骨髓組織施行過此技術,MSC細胞至少可以培養5個繼代。以ISCT2006公布的定義分析cell marker CD105,73,90>95%,CD45,34<2%,且具osteoblasts,adipocytes,chondrocytes的分化能力,顯示為MSC細胞。

應用範圍

由骨髓組織取得人類間質幹細胞,作為後續生醫相關技術開發之細胞來源。

接受技術者具備基礎建議(設備)

P2實驗室,細胞培養設備

接受技術者具備基礎建議(專業)

具細胞培養經驗者

技術分類 複合醫材

聯絡資訊

聯絡人:陳婉昕 再生醫學技術組(0R000)

電話:86-3-5912810 或 Email:WhsinChen@itri.org.tw

客服專線:+886-800-45-8899

傳真:+886-3-5820369

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